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Image Search Results
Journal: Cell Reports Medicine
Article Title: Ancestral library identifies conserved reprogrammable liver motif on AAV capsid
doi: 10.1016/j.xcrm.2022.100803
Figure Lengend Snippet: Library production and in vitro infectivity (A) A barcoded AAV vector library was produced either with (N = 5) or without (N = 3) addition of an exogenous assembly-activating protein (AAP). Individual dots in MA plots represent distinct barcodes, colors represent the amino acid identity at position 5. To the right of MA plots, eCDF plots with SEM depicted as horizontal error bars. (B) Results of an independent sites linear elastic net regularization approach show that addition of AAP modifies the impact of certain sites on production of vector. (C) Huh7 cells were transduced with the barcoded AAV library and DNA/RNA were isolated from those transduced cells (N = 5 per condition). Individual barcodes in MA plots are colored by identity at position 3 within the library. Extant barcoded vectors were also spiked into this transduction mixture. Fold change is plotted as bar graphs (error bars determined by bootstrapping, 1,000 replicates). (D) Regularization and linear modeling approach reveal potential similarities and differences among our positions of variation with respect to transduction and gene expression in vitro .
Article Snippet:
Techniques: In Vitro, Infection, Plasmid Preparation, Produced, Transduction, Isolation, Gene Expression
Journal: Cell Reports Medicine
Article Title: Ancestral library identifies conserved reprogrammable liver motif on AAV capsid
doi: 10.1016/j.xcrm.2022.100803
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Plasmid Preparation, Recombinant, Imaging, RNA Sequencing, Software, Microscopy